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Multiplexing Immunofluorescence

Quantitative pathology services through multiplexed IF

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Multiplex IF Reveals Complex Tissue Biology

Multiplex Immunofluorescence (mIF) enables simultaneous visualization of multiple protein or RNA biomarkers within a single tissue section, a powerful technique revealing the spatial relationships between different cell populations. Fluorescent approaches allow you to go beyond labeling two or three analytes simultaneously using chromogenic approaches, enabling a deeper understanding of the tumor microenvironment and cellular interactions in both research and clinical contexts.

Why Partner with Lanterne Dx (LDX)

At Lanterne Dx (LDX), our scientists, histotechnologists, and pathologists collaborate to deliver end-to-end multiplex assay development. Our team’s expertise spans biomarker identification, tissue selection, IHC optimization, fluorescent monoplex validation, and final multiplex assembly, ensuring every stage is guided by experienced professionals. We understand the critical progression—from selecting the right tissue and optimizing antibodies to building a balanced, high-performing multiplex fluorescent assay. 

Our mIF offerings include

  • Off-the-shelf panels with validated biomarkers
  • Fully customized multiplex panels tailored to your study
  • High-resolution imaging and quantitative analysis for data-rich insights

 

Example 6 Color Panel Built with Opal Technology

The images below illustrate a six-plex panel including PD1, FoxP3, PDL1, PanCK, CD68, and CD8, scanned on the PhenoImager Fusion platform. Together, they create a composite image visualizing six biomarkers on one tissue slide, offering a powerful window into the tumor microenvironment.

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